Users should refer to the original published version of the material for the full abstract. The hydrodynamic radius (Stokes radius) as measured by sedimentation velocity or dynamic light scattering is that of an equivalent sphere and contains. No warranty is given about the accuracy of the copy. However, users may print, download, or email articles for individual use. Instruct has 0 centres offering Multi Angle /. Detailed biophysical characterization of biomolecules in solution. Copyright of Journal of Nanoparticle Research is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. Multi Angle / Dynamic Light Scattering (MALS).These features make it an ideal tool to support both development and quality control of particle materials for a broad range of applications. Overall, we show that MADLS® is a rapid and straightforward method for the reproducible measurement of particle concentration, as well as size, requiring minimal sample preparation, without the need to calibrate using a pre-determined concentration series, and applicable to a broad range of materials. A linearity of within 40% across a concentration range of 3♱08 to 2♱011 mL−1 for concentration measurements was also demonstrated by using gold nanoparticles and gravimetric dilutions for method validation. The repeatability of the measurements, in terms of relative standard deviation, is typically below 30%. Here it is shown that the level of accuracy that can be expected for the concentration of particles is typically within 50% of the nominal value across a range of materials and sizes and, for some samples, within 20%. We show that the concentration working range depends on the material's optical properties, size and concentration. Our apparatus uses standard, readily available optomechanical components. In this paper, a novel successive updating of the angular weighting (AWSU) method is proposed. We describe a light scattering apparatus based on a novel optical scheme covering the scattering angle range 0.5dg le le 25dg, an intermediate regime at the frontier between wide angle and small angle setups that is difficult to access by existing instruments. However, determining the weighting coefficient is affected by the noise in the measured MDLS data. We evaluate the method accuracy, linearity and reproducibility, as well as the operational nanoparticle concentration and size range. Abstract The angular weighting coefficient is key to accurate particle size distribution (PSD) measurement using multiangle dynamic light scattering (MDLS).
We describe the theory of the method and its application to nanoparticles made of gold, silica and polystyrene, with diameters ranging from 30 to 400 nm, and demonstrate some of the limitations with particles of sizes 500 nm and above.